The Metallo laboratory at the Salk Institute for Biological Studies applies a multi-disciplinary approach to understand how metabolism drives disease states and uses this information to improve people’s health and wellness. We incorporate analytical chemistry, mass spectrometry, cell biology, and systems-based modeling to study metabolic regulation in cells, tissues, animals, and people. We integrate the use of stable isotope tracers and engineering principles to quantitatively describe how metabolic function (or dysfunction) contributes to human disease.